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Column Volume Calculator

Column Volume Calculator | Chromatography, HPLC, FPLC & Process Columns

Calculate column volume, bed volume, and residence time for any chromatography column using our global column volume calculator.

0.1–1000 mm
10–2000 mm
0.3–0.95
0.01–10,000 mL/min
Select unit
Affects defaults

Enter column dimensions to calculate volume.

Master column calculations with the column volume calculator.

Geometric Volume

V = Ο€ Γ— (d/2)Β² Γ— L

Total bed

Void Volume

V_void = V Γ— Ξ΅

Mobile phase

Residence Time

t = V_void / F

Minutes

V = Ο€rΒ²h | 1 CV = 1 column volume

Column Volume Calculator: Global Chromatography Tool

The most accurate column volume calculator for HPLC, FPLC, process columns, and lab-scale chromatography β€” with void volume and residence time.

"Volume is the heart of separation." β€” Chromatography Principle

How the Column Volume Calculator Works

Our column volume calculator uses standard formulas:

  • Geometric Volume: V = Ο€ Γ— (d/2)Β² Γ— L
  • Void Volume: V_void = V Γ— Ξ΅
  • Residence Time: t = V_void / F (min)
  • 1 CV = 1 full column volume

Understanding Column Volume Calculator Results

You get:

  • Total Bed Volume (mL, L, Β΅L)
  • Void Volume (mobile phase)
  • Residence Time (minutes)
  • CV per mL of flow

Why Use This Column Volume Calculator?

Trusted by scientists worldwide:

  • All column scales
  • Porosity & flow rate
  • Real-time validation
  • Residence time
  • Mobile-optimized

Real Column Volume Calculator Examples

  • 4.6 Γ— 250 mm HPLC: β†’ 4.15 mL total, 2.9 mL void
  • 10 Γ— 300 mm Prep: β†’ 23.6 mL total
  • 50 Γ— 500 mm Process: β†’ 981 mL total
  • 1 mL/min flow: β†’ 2.9 min residence

Typical Porosity Values

  • C18 Silica: 0.65–0.75
  • IEX Resin: 0.70–0.90
  • Agarose: 0.85–0.95
  • Monolith: 0.80–0.90

Common Column Sizes

  • Analytical: 2.1–4.6 mm ID, 50–250 mm
  • Preparative: 10–50 mm ID, 100–300 mm
  • Process: 100–1000 mm ID, 200–1000 mm

Get Started with Column Volume Calculator

  1. Enter ID & Length
  2. Set Porosity
  3. Enter Flow Rate
  4. Select Output Unit
  5. View Instant Results

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The leading column volume calculator for chromatography labs. Start now.

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